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KASP genotyping protocols for your qPCR instrument

Performing KASP™ genotyping in your own lab is easy. You need your DNA samples, your ordered KASP assays, universal KASP-TF Master Mix from LGC and a FRET-capable plate reader or a qPCR instrument.

It is straightforward to perform KASP™ genotyping in your own laboratory. Once you have your DNA samples, and have ordered your SNP-specific KASP assays and universal KASP-TF Master Mix from Biosearch Technologies, all that is required to read the fluorescent signal from completed KASP reactions is a FRET-capable plate reader or a qPCR instrument. 

The table below outlines the optimal ROX level of KASP-TF Master Mix for the most commonly used qPCR instruments. Where applicable, a manual for running KASP on the specific qPCR instrument can also be downloaded.

KASP-TF Master Mixes are formulated without Triton™ X-100. For FRET-capable plate readers, Biosearch Technologies recommends initial trials with Standard ROX KASP-TF Master Mix. Run one of our free-of-charge trial kits on your qPCR instrument before running your own DNA samples and KASP assays.

Please note that KASP is currently available for endpoint genotyping only. 

PCR/qPCR instrument KASP-TF Master Mix for optimal results KASP-TF Master mix 
product codes		 KASP genotyping protocol
ABI 5700 High ROX

KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)

 Please enquire
ABI 7000 High ROX KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)		 Please enquire
ABI 7300 High ROX KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)		 Please enquire
ABI 7500, 7500 Fast Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download
ABI 7700 High ROX KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)		 Please enquire
ABI 7900, 7900HT, 7900 HT Fast High ROX KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)		 Download
ABI QuantStudio 3K and 5K Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Please enquire
ABI QuantStudio 6K and 7K Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download
ABI QuantStudio 12K Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Please enquire
ABI StepOne, StepOne Plus High ROX KBS-1050-131 (2.5 mL)
KBS-1050-132 (25 mL)
KBS-1050-133 (250 mL)		 Download
ABI ViiA7 Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download
Agilent (Stratagene) MX3000, MX3500P Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download
Agilent (Stratagene) MX4000P Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Please enquire
BIO-RAD iCycler iQ, MyiQ, MyiQ2* Standard ROX*

KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)

 Please enquire
BIO-RAD CFX-96, CFX-384* Standard ROX* KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Download
Nexar™ Standard ROX*

KBS-1050-201 (2.5 mL)
KBS-1050-202 (25 mL)
KBS-1050-204 (250 mL)

 Download
IntelliQube™ Standard ROX* KBS-1050-201 (2.5 mL)
KBS-1050-202 (25 mL)
KBS-1050-204 (250 mL)		 Download
Fluidigm KASP Fluidigm mix (Standard ROX)

KBS-1050-145 (2.5 mL)
KBS-1050-146 (25 mL)

 Please enquire
Illumina Eco RT Standard ROX* KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Download
Qiagen RotorGene Q Standard ROX KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Download
Roche Lightcycler  2.0*  Standard ROX* KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Please enquire
Roche Lightcycler 480* Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download 
Filter set information
 
Roche Lightcycler 480 II (v2)* Low ROX

KBS-1050-121 (2.5 mL)
KBS-1050-122 (25 mL)
KBS-1050-123 (250 mL)

 Download  
Filter set information
 
Roche LC96* Standard ROX* 
NOTE: Data must be exported to 
alternative software for cluster analysis.		 KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Download (factsheet only)
 
Thermo Scientific PikoReal* Standard ROX KBS-1050-101 (2.5 mL)
KBS-1050-102 (25 mL)
KBS-1050-103 (250 mL)		 Download
SNPline™ instrumentation Standard ROX KBS-1050-103 (250 mL) (96/384-well plates)
KBS-1050-113 (250 mL) (1536-well plates)		 Please enquire

*For these instruments, we recommend Standard ROX KASP Master mix although the instruments will work with both low and high ROX mixes. These instruments can read ROX but the instrument-specific software does not use ROX values for normalisation. Running KASP with standard ROX KASP Master mix gives the user the option to export data to alternative software to perform normalisation if required.