SARS-CoV-2 whole genome sequencing service for variant detection
As the COVID-19 pandemic continues, the virus responsible, SARS-CoV-2, continues to spread and mutate, resulting in the emergence of novel variants that could have important consequences in the fight against this virus. Some variants may spread more easily, while others could cause more serious disease. In order to keep up with this virus and continue our fight against it, it is critical that we identify and track both known and novel SARS-CoV-2 variants.
The best and least biased method for variant identification and tracking is SARS-CoV-2 whole genome sequencing. While this method is optimal for variant identification and tracking, whole genome Next Generation Sequencing is complicated and requires both extensive expertise, resources and instrumentation.
To help alleviate the strain on current Next Generation Sequencing laboratories and provide access to sequencing resources that many researchers may not have, LGC, Biosearch Technologies offers SARS-CoV-2 whole genome sequencing and variant identification service.
Taking the strain and worry from you and providing the sequence data you need to continue the fight against COVID-19.
Service Specification
| Starting material | Isolated, purified RNA – customer provided. No primary COVID-19 samples are accepted. |
|---|---|
| cDNA synthesis | LGC converts purified RNA into 1st strand cDNA |
| SARS-CoV-2 library production | LGC produces overlapping amplicon library with consistent, uniform coverage across the genome |
| Sequencing | LGC performs sample processing, QC and Illumina sequencing |
| Bioinformatics | LGC performs whole genome assembly and variant identification using Wuhan-1 strain as reference. A variant report as an easy-to-understand, fully annotated spreadsheet as well as in VCF format are delivered. A full-length, assembled consensus genome sequence is also provided for each sample. |
| Turnaround time | 10 business days |
| Application | Research use only (RUO). Not for use in diagnostic procedures. |
Not included in the service
- Viral RNA purification from primary samples
- Any diagnostic analysis or interpretation
Regional availability
Our SARS-CoV-2 whole genome sequencing service is offered through our Berlin NGS service lab and is currently available to customers in Europe, the Middle East and Africa. Please enquire if you have questions about availability in your region.
For Research Use Only. Not for use in diagnostic procedures.