Agricultural NGS technologies and services
Next generation sequencing (NGS) is a vital tool for plant and animal breeding programmes, but its complexity and cost can be challenging. At LGC Biosearch Technologies, we simplify NGS for agrigenomics with cost-effective products and services.
Our technologies enable high-throughput DNA analysis in plant, livestock, and aquaculture genetics, supporting more productive, resilient crops and animals, as well as food safety and animal health. We help leading organizations improve farming productivity sustainably through genomics, and we're ready to assist you in achieving your breeding goals with NGS.
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Check new extraction and genotyping workflow configuratorHigh-throughput genotyping solutions
| KASP | Amp-Seq One | Flex-Seq | Capture-Seq | nGBS & ddRAD | WGS | |
|---|---|---|---|---|---|---|
| Technology | PCR (fluorescent readout) | Targeted genotyping by sequencing (GBS) based on amplicon sequencing | Targeted GBS based on probe hybridisation (NGS readout) | Targeted GBS based on in-solution hybridisation-based capture (NGS readout) | Restriction enzyme-based genotyping by sequencing (NGS readout) | Whole genome sequencing (NGS readout) |
| Technical applications | Targeted genotyping | Targeted genotyping | Targeted genotyping | Targeted genotyping | Targeted genotyping | Genome exploration |
| Agricultural biotechnology applications | QA/QC, MAS, MABC, Parentage | MAS, genomic prediction, parentage | Genotyping, genomic prediction, haplotyping, MAS, MABC, parentage | Genotyping, haplotyping, genomic prediction, marker discovery, insertion site characterisation, GWAS | Marker discovery (untargeted) | Full genome marker discovery, allele mining, skim sequencing, structural analysis |
| Upper marker number density | Hundreds | Thousands | Tens of thousands (genotyping) | Hundreds of thousands (genotyping) | Tens of thousands (discovery) | Whole genome |
| Pre-defined content | Human, rat, mouse, wheat, maize, tomato and others | - | Several, e.g., Blueberry, Cattle, Corn, Cotton, Pig, Soybean | - | - | - |
| Min. sample number | 1,536+ | 48+ | 96+ | 96+ | 12+ | 12+ |
| Kit/service | Master mix, assays and Instruments | Reagent system + analysis pipeline | Service | Service | Service | Service |
| Lab service sites | Hoddesdon (UK) | N/A | Berlin (DE), Middleton (US) | Middleton (US) | Berlin (DE) | Berlin (DE), Middleton (US) |
| Input DNA requirement | Low quality/crude | Low quality DNA compatibility options | Medium to high quality | Medium to high quality | High quality | High quality |
| Default service TAT* | 2 weeks | - | 4-6 weeks | 6-8 weeks | 6-8 weeks | 8-12 weeks |
| Default protocol TAT** | 80-90 minutes | 180-240 minutes | - | - | - | - |
* Turnaround time excl. assay design, DNA extraction and 4–6 weeks panel design
** excl. assay design, DNA extraction and sequencing; fast turnaround time options available
Targeted GBS kits and services for genomic selection
Targeted sequencing technologies are ideal for high-throughput breeding programmes to achieve genetic gains with flexibility of content and de-novo SNP and structural variation analysis that is not possible with microarray platforms.
LGC’s targeted genotyping by sequencing technologies are ideally suited for a wide range of marker densities from tens to hundreds of thousands.
- Amp-Seq One – 1-step targeted genotyping by sequencing kits up to thousands of markers
- Amp-Seq – low to medium density targeted genotyping by sequencing kits and workflow
- Flex-Seq – medium density targeted genotyping by sequencing service
- Capture-Seq – medium to high density targeted sequencing service
Library preparation for whole genome sequencing (WGS)
Whole genome sequencing (WGS), resequencing (WGR), and skim sequencing are powerful techniques for uncovering population diversity and characterising structural variations in the genome. But getting accurate and reliable data is highly dependent on the quality of the input library. With NxSeq™ DNA fragment library preparation kits you can reliably generate highly complex and informative libraries for uniform and unbiased sequencing data that is critical for uncovering important diversity associated with phenotypically relevant traits.
Genotyping by sequencing (GBS) services for SNP discovery and GWAS
GBS is the SNP discovery method of choice when reference genome sequence is unavailable. It is ideal for simultaneous genotyping of multiple DNA samples and scoring random markers across an entire genome. Get all the benefits of GBS whilst keeping your team focused on the biology of crop improvements with Biosearch Technologies’ all-inclusive GBS services. Our scientists have deep expertise in techniques ranging from normalised GBS (nGBS), double digest RadSeq (ddRAD) and other GBS complexity reduction sequencing approaches and are ready to work with your team to connect phenotypically relevant traits to genetic markers to accelerate your marker-assisted breeding programs using GBS.
Other NGS laboratory services
Our all-inclusive NGS laboratory services can keep your workflows lean and agile, delivering the flexibility to address over-capacity, one-off projects, and scale-up to full production pipelines. With labs in Europe and the US, our services are freed from geographic constraints.
We offer a range of bespoke NGS services including:
- 16S/18S rRNA sequencing
- Target enrichment methods
- Metagenomics
- Custom sequencing projects
Our experts are ready to collaborate with you on selecting and executing the most effective methods for obtaining actionable data to advance your science.
Enzymes for sequencing applications
Accurate NGS data and high-quality libraries are reliant on enzymes that are highly specific and deliver reproducible results. Our extensive portfolio of library preparation enzymes covers both workhorse and high-quality specialty enzymes that are compatible with a variety of DNA and RNA sequencing methods, giving you the maximum flexibility to tailor library preparation to your specific research problem whilst maintaining data quality.
Plant and animal sample preparation for NGS
Because sample quality affects the accuracy and reliability of next generation sequencing (NGS) and PCR, getting sample preparation right is critical. Our high high-performance DNA and RNA extraction and purification kits, automated DNA purification instruments and tailor-made extraction services deliver high quality DNA and RNA that improves your data quality and enables you make critical research decisions.
Large scale genotyping programs require efficient tools for high-density SNP discovery whilst keeping costs contained. Here we present an optimised, self-tuning genotyping by sequencing (GBS) method, called normalised GBS (nGBS), which efficiently reduces the genome complexity of any species to a few hundred thousand loci across the complete genome.
In this application note, we explain how the use of genomics for selection can accelerate livestock breeding programmes. We describe why Flex-Seq’s hybridised dual probe targeted genotyping by sequencing platform animal panels are the panel of choice for many breeders.
Amp-Seq, the new ultra-fast, high-throughput, targeted genotyping by sequencing workflow where purification with sbeadex Lightning takes up to 5 minutes, and the entire workflow delivers results in 24 hours. This new industrial scale high-speed, high-accuracy mid-plex targeted NGS workflow addresses the issues faced by many breeders who need to analyse thousands of samples in a short time frame.
