SNP marker development for a genome screening tool is based on three steps:

• Detection
• Validation
• Selection.

Detection requires identifying a large pool of SNPs in the species of interest. The objective is to maximise the opportunities for detecting SNPs, while ensuring that the SNPs will be suitable for their application for screening.

Validation is necessary for informive SNP assay development, in order to maximize the number of functional polymorphic markers in genetic segregation analysis. Validation is often based on a subset of the detected SNPs.

Crossing populations and economically significant varieties or breeds can be used to confirm and validate informative KASP SNP markers (98-100% conversion rate). Assessment can involve 20 to 200 varieties with up to 5000 assays.

SNP validation in action

LGC is helping to shape agrigenomics research – in fact KASP™ has over 1,000 published references across a wide variety of applications.

Increasing wheat yields is now one of the top priorities for agricultural research and the sustainable intensification of global agriculture. With the use of KASPgenotyping technology there is now a way to increase the yield of wheat in an efficient and stable manner without compromising on food safety.

The LGC wheat genotyping panel, developed in conjunction with the University of Bristol, School of Biological Sciences UK, contains over 7,000 functionally validated SNP assays that breeders and scientists can use to enable the development of precision breeding in wheat hybrids. The panel offers pre-validated assays thus facilitating cost benefits, guaranteed performance and fast delivery.