qPCR application chart
For guidance on mapping probe choice to common qPCR/PCR applications.
A one-size fits all approach to designing qPCR and genotyping assays does not account for different experimental goals and probe sequence requirements such as percent GC content, secondary structure avoidance, target Tm values and more. Where one probe mechanism renders it challenging to design around a difficult target sequence, another will overcome these challenges (e.g. maintaining specificity or quenching efficiency) without compromising results. At LGC Biosearch Technologies we supply you with a toolbox of various chemistries and probe formats so that you can realise your best results with less effort while saving time and precious sample.
|BHQplus™||BHQnova™||BHQ™ Dual-labeled Probes||KASP™ genotyping chemistry||Minor groove binder (MGB)|
|SNP genotyping/allelic discrimination||✔||✔||✔||✔|
|Gene expression analysis||✔||✔||✔||✔||✔|
|Pathogen detection/viral load quantification||✔||✔||✔||✔||✔|
|AT rich templates||✔||✔||✔||✔||✔|
|Copy number variation||✔||✔||✔||✔||✔|
|Polyploid genotyping, plants||✔||✔||✔|
|Long probe sequence||✔|
How to order
- Design your probe using RealTimeDesign™ Software. Access the software programme and download a copy of our quick start guide here.
- Use the spectral overlay tool to choose the right dye and fluorophore combination for your instrument.
- Verify that the dyes (fluorophore and quencher) are spectrally distinct enough for the signals not to bleed through into each other.
- Select the appropriate probe format according to sample type and application
To order, locate the "Order" button on the corresponding probe product page and follow the instructions.