Nexar Inline Liquid Handling System

Nexar™is a modular Inline Liquid Handling and Assay Processing System offering a highly scalable solution for PCR-based genotyping.

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Pipette Dispensing

The Dispense Pipette features a CyBio head with 384 or 96 tips. It aspirates the samples from the sources plate and stamps them in the Array Tape. The dispense jet aspirates the reagent from the source plate and adds it to the samples on the fly. Aspirate and dispense volumes, along with CVs, are determined by the type and style of pipette and tip tray used.

Fluid Handling Chart

Product	Parallel channel	Independent, multi-channel	Number of channels				Nexar validated volume range	Nexar CVs	Manufacturer validated volume range
Product	Parallel channel	Independent, multi-channel	4	8	96	384	Nexar validated volume range	Nexar CVs	Manufacturer validated volume range
CyBio	X				X	X	600 nL to 800 nL	< 6.6% with 10 µL tips > 4.7% with 25 µL tips	0.2 - 25 µL
Dispense Jet		X	X				750 nL to 1,250 nL	< 7%	NA
FlexJet™			X	X			250 nL to 2,500 nL	< 7%	NA

Lab Adaptable Modularity

The Nexar and Araya™ are comprised of interchangeable modules. The result is a best-in-class, plug and play, modular platform designed to fit your processes. Your investment will be protected as the system adapts to your evolving laboratory requirements. This modularity allows you to embrace change with simplicity, seamless operational impact, and minimal cost.

Nexar In-Line Liquid Handling And Assay Processing System Upgrades

Plate Management
Dispense Pipette and Wash Station
Dispense Jet
Dryer
Incubator
Inline Detection
Sealer

Genotyping application	Array Tape™ Process on Nexar™, Soellex™ and Araya™
Build assay	The Nexar dispenses 0.8 µL sample and 0.8 µL reagent in the equivalent of 277 384-plates in a continuous strip of Array Tape
Process	Entire reel of Array Tape is sealed and loaded into the Soellex water bath for approximately 90-120 minutes
Dry	The reel of Array Tape is dried (optional on a custom centrifuge)
Detection	The Araya scans the entire reel at a rate of 28 seconds per 384-well array
Plates/day	400 arrays (384 wells)
Data points/day	153,600
Processing time	8 hours

CyBio Dispense Pipette and Wash Station

Customizable Rapid Dispense Pipette

The Dispense Pipette contains an integrated CyBio Light Pipetting Head. It can be customized with a 96 or 384 tip tray. Aspirate and dispense volumes, along with CVs, are determined by the type and style of pipettor and tip tray used. Typically, a large volume of sample is aspirated and a smaller volume is dispensed simultaneously into each well in the array. The Dispense Pipette Attachment also contains a 1D/2D barcode reader that can read the 2D code on the Array Tape and the 1D (linear) code on the end of the source plate.

Parallel channel pipetting from plates using the CyBio LDH head provides rapid sample and reagent dispense into Array Tape using 96- or 384-tips in either full or quadrant stamping in a range of 600 nL to 800 nL.

Product	Parallel channel	Number of channels		Nexar validated volume range	Nexar CVs	Manufacturer validated volume range
Product	Parallel channel	96	384	Nexar validated volume range	Nexar CVs	Manufacturer validated volume range
CyBio	X	X	X	600 nL to 800 nL	< 6.6% with 10 µL tips > 4.7% with 25 µL tips	0.2-25 µL

Pipetting head specifications

CYBIO 384 HEAD (CYBIO)

Mechanism: Simultaneous air displacement
Manufacturer Suggested Volume Range: 0.2-10 µL/25.0 µL (dependent on tip vol.)
Manufacturer Precision: CV < 2%, at 1.0 µL

CyBio Dispense Pipette Wash Stations

Biosearch Technologies offers two dispense pipette washing options to meet tip-cleaning needs and minimize plastic waste.

Pipette Wash

The Pipette Wash defends against cross-contamination while minimizing contaminant carryover between plates. During normal analytical runs, the wash procedure is automatically controlled by the Nexar’s built-in software and/or protocols.

Sonic Wash

The Sonic Wash provides ultrasonic washing of pipette tips to clean residual samples from nozzles and pipette tips. Water is plumbed into the tub and overflows the edges into a collection trough to be pumped out of the instrument. Ultrasonic cleaning is provided by a transducer mounted under the tub while water in the tub is aspirated and flushed out multiple times to remove residual sample.