Posters & Presentations

Alternative polyadenylation of eukaryotic initiation factor 5A-2 (eIF5A-2) mRNAs examined by quadruplex single molecule RNA fluorescence in situ hybridization (smRNA-FISH). Johansson, H.E., Yin, R.H., Mandal, S., and Park, M.H. Presented at the AACR Annual Meeting 2016, New Orleans, LA, April 16-20, 2016.

EML4-ALK pre-mRNA and mature mRNA fusion detection using RNA fluorescence in situ hybridization (RNA FISH). Coassin, S.R.; Kim, B.-C.; and Hans E. Johansson, H.E. Presented at the AACR Annual Meeting 2015, Philadelphia, PE, April 18-22, 2015.

Duplex imaging of pre-lncRNAs and mature lncRNAs by Stellaris® RNA fluorescence in situ hybridization (RNA FISH). Orjalo A.V., Jr., and Johansson, H.E. Presented at the Keystone Symposium on Long Noncoding RNAs: From Evolution to Function, Keystone, CO, March 15-20, 2015.

Stellaris® RNA fluorescence in situ hybridization (RNA FISH) for the detection of long non coding RNA biomarkers. Orjalo A.V., Jr., and Johansson, H.E. Presented at the AACR Conference on Translation of the Cancer Genome, San Francisco, CA, Feb. 7-9, 2015.

Detection of lncRNA implicated in disease by Stellaris® RNA Fluorescence in situ hybridization (RNA FISH) (PDF 615KB) Coassin S.R.; Yin R.H.; Johansson H.E.; Orjalo A.V.; Presented at EMBL 2014 in Heidelberg, Germany, June 2014.

Detection and validation of novel RNA cancer biomarkers by single molecule RNA fluorescence in situ hybridization (smRNA FISH) (PDF 611KB) Johansson, H.E.; Orjalo, Jr.A.; Coassin S.R.; Yin R. Presented at AACR 2014 in San Diego, CA, April 2014.

Imaging of Disease Relevant lncRNAs by Stellaris® RNA fluorescence in situ hybridization (RNA FISH) (PDF 1.05MB) Johansson, H.E.; Orjalo, Jr.A.; Coassin S.R. Presented at EMBO|EMBL Symposium: The Non-Coding Genome in Heidelberg, Germany, October 2013.

High-throughput imaging and high content analysis of disease relevant lncRNAs examined by RNA fluorescence in situ hybridization (RNA FISH) (PDF 633KB) Johansson, H.E.; Orjalo, Jr.A.; Coassin S.R.; Ruth J.L.; Stossi F.; Mancini M.A. Presented at AACR 2013 in Washington, DC, April 2013.

Improving dye brightness and photostability in Stellaris® RNA FISH assays (PDF 1.25MB) Johansson M.K.; Coassin S.R.; Orjalo Jr.A.; Lyttle M.H.; Cook R.M. Presented at ABRF 2013 in Palm Springs, California, March 2013.

Detection of long noncoding RNA in cancer by fluorescence in situ hybridization (PDF 577KB) Orjalo, Jr.A.; Coassin, S.R.; Johansson, H.E.; Ruth J.L. Presented at the Keystone Symposium in Development and Cancer in Vancouver, BC, Canada, January 2013.

Hypusine pathway gene expression examined by single molecule RNA fluorescence in situ hybridization (smRNA-FISH) (PDF 432KB) Johansson, H.E.; Orjalo, Jr.V.; Dadhich, S.; Park, M.H. Presented at AACR 2012 in Chicago, Illinois, April 2012.

Stellaris™ fluorescence in situ hybridization (FISH) probes: a powerful tool for mRNA detection (PDF 4.4MB) Orjalo, Jr. A.; Johansson, H.E.; Ruth, J.L. Presented at CAP 2011, Grapevine, Texas, September 2011.

The BTI1 azoreductase colorimetric and fluorometric reporter system (PDF 912KB) Johansson, M.K.; Wong, A.C.; Armstrong, E.S.; Peterson, E.J.; Grant, R.E.; Reddington, M.V.; Cook, R.M.; Johansson, H.E. Presented at Experimental Biology 2009, New Orleans, Louisiana, April 2009.

Oligo Design Across the Mouse Genome (PDF 3.5MB) Sowers, B.A.; Le, L.; Cook, R.M. Presented at qPCR 2009, Freising, Germany, March 2009.

A Rapid Bioinformatic Engine for Multiplexed qPCR Design (PDF 4.4MB) Sowers, B.A.; Le, L.; Cook, R.M. Presented at qPCR 2009, Freising, Germany, March 2009.

Genotyping with BHQplus™ - Fortified Probes Achieve Powerful Discrimination (PDF 1.8 MB) Sowers, B.A.; Messenger, V.E.; Cook, R.M. Presented at qPCR Symposium USA, Palo Alto, California, October 2007.

Intricacies of PCR Multiplexing As Revealed Through a Pathogen Detection Assay (PDF 1.7 MB) Messenger, V.E.; Sowers, B.A.; Cook, R.M. Presented at qPCR 2007 Event: 3rd International qPCR Symposium & Industrial Exhibition & Application Workshop, Freising-Weihenstephan, Germany.

RealTimeDesign™ - Free Web-based Software For Dual-labeled and Amplifluor® Design (PDF 1.4 MB) Sowers, B.A.; Songster, M.F.; Smith, K.E.; Snyder, D.L.; Cook, R.M. Presented at 20th Anniversary San Diego Conference DNA Probe Technology, San Diego, CA, March 2006.

RealTimeDesign™ Software - An Advanced Web-based Program for Real-time PCR Sequence Design (PDF 1.4 MB) Sowers, B.A.; Songster, M.F.; Snyder, D.L.; Smith, K.E.; Cook, R.M. Presented at 2nd International qPCR Event, Freising-Weihenstephan, Germany, September, 2005.

Pulsar-Z: A New Cell-Permeable Dye for Cell Cycle Analysis Using Flow Cytometry and Laser Scanning Cytometry at 488 nM (PDF 494 KB) Tighe, S.W.; Johansson, M.K.; Resnikoff, J; Cook, R.M. Presented Montpellier, France, May, 2005.

Optimization of Probe Performance in Real-Time PCR through an Understanding of Synthesis Impurities. (PDF - 4.1 MB) Sowers, B.A.; Peterson, E.J.; Grant, R.E.; Lin, W.Y.; Dick, D.J.; Cook, R.M. Presented at Quantitative PCR, San Diego, CA, March, 2005.

CAL Fluor® Dyes: Multiplexing Reporters for Real-Time PCR Instruments and Probe Designs (PDF - 1.3 MB) Sowers, B.A.; Reddington, M.; Lyttle, M.; Johansson, M.K.; Cook, R. Presented at Quantitative PCR, San Diego, CA, March 2004.

BHQs Efficiently Quench a Wide Variety of Fluorophores Including IR Dyes, Lanthanides and Ru Complexes. Johansson, M.K.; Lyttle, M.; Carter, T.; Dick, D.; Cook, R. Presented at CHI's Profiling PCR and Beyond, Washington, D.C., June, 2002.

Development and Evaluation of Passive Reference Candidate Compounds for Normalization of Signal in Real-Time PCR. Potter, C.; Johansson, M.K.; Dick, D.; Cook, R. Presented at ABRF 2002: Biomolecular Technologies: Tools for Discovery in Proteomics and Genomics, Austin, TX, March, 2002; poster P13-S.

Development of Ru(bpy)₃ CPG for Solid-Phase Synthesis of 3'-Labeled Oligonucleotides. Johansson, M.K.; Lyttle, M.H.; Cook, R.M. Presented at TIGR's 13th International Genome Sequencing and Analysis Conference, San Diego, CA, October, 2001; poster P080.

Fmoc Chemistry Compatible Methods for Thio-Ligation Assembly Of Proteins. Biancalana, S.; Hudson, D.; Songster, M.F.; Thompson, S.A. Presented at the 17th American Peptide Symposium, San Diego, CA, June, 2001; poster P157.

New Quencher Derivatives for Fluorescent Quenching Peptide Assays. Walton, T.A.; Dick, D.J.; Hudson, D.; Johansson, M.K.; Lyttle, M.H.; Songster, M.F.; Cook, R.M. Presented at the 17th American Peptide Symposium, San Diego, CA, June, 2001; poster P158.

Alternatives to FRET: Design of Fluorescence-Quenched Oligonucleotide Probes with Extremely High Signal/Noise Ratios. Johansson, M.K.; Cook, R.M.; Dick, D.; Lyttle, M. Presented at TIGR's 12th International Genome Sequencing and Analysis Conference, Miami, FL, September, 2000; poster P080.

New PEG-PS Resins for Solid-Phase Organic Synthesis. Adams, H.A.; Hudson, D.; Steinauer, R.; White, P. Presented at CHI's 4th Annual High-Throughput Organic Synthesis Symposium, San Diego, CA, February, 1999; poster 1.

Improved Synthesis Procedures for Aminomethyl- and MBHA-Functionalized Polystyrene Resins. Lyttle, M.H.; Hudson, D.; Adams, H.A.; Jammalamadaka, V.; Songster, M.F.; Cook, R.M. Presented at the 2nd Lake Tahoe Symposia on Molecular Diversity, Lake Tahoe, CA, January, 1998.