How KASP genotyping works

Scientists around the world have been using KASP™ genotyping assays to transform commercial agriculture for more than two decades, making food production more sustainable and improving crop yields. This proprietary technology enables fast and reliable genotyping of large to small samples of any species.

KASP genotyping assays are based on competitive allele-specific PCR and enable bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci. KASP has been cited in thousands of peer reviewed scientific publications for hundreds of species with millions of KASP markers identified.

The wonderful simplicity of KASP genotyping assays

A KASP genotyping reaction has three components:

SNP-specific KASP assay targeting primers (KASP Assay Mix)
Universal KASP-TF Master Mix
Template DNA

Simply combine the three components, run the genotyping assay using a thermal cycler and complete an end-point fluorescent read. KASP delivers > 99.8% accuracy and >90% SNP and Indel assay conversion rate. Completed KASP reactions are readable on most FRET-capable plate readers and qPCR instruments.