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IntelliQube Automated PCR Instrument

Multiplex changing genetic target markers on the fly for pennies per sample with the world’s most efficient, fully integrated qPCR workflow system in a box.

Unlocking deeper market knowledge from multiple types of genetic markers with real-time qPCR is now more powerful than ever.

Generate answers quickly and flexibly with the world’s only fully automated high-throughput multiplexing qPCR system. Using Array Tape™ technology, the IntelliQube automates assay preparation, plate sealing, qPCR cycling and detection in a multiplexing system. Combine with proprietary BHQPlex CoPrimers technology for assay interchangeability, making it the most flexible high sample number qPCR system available.

Agrigenomics applications

  • Copy number variation
  • Adventitious presence
  • Quality testing
  • GMO detection
  • SNP genotyping

Genetic screening applications

  • Pharmacogenomic testing
  • Testing for disease predisposition
  • Ancestry testing

For Research Use Only. Not for use in diagnostic procedures.

IntelliQube qPCR system

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IntelliQube™ Liquid Handling

IntelliQube leverages best-in-class, multi-functional dispensing for high-speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBio® FeliX 96- or 384-channel pipetting head. Reagent dispensing is performed by the Dispense Jet, a 4-channel, non-contact dispense head that loads the assay mixtures required for your protocols. With CVs less than 5%, the liquid handling on the IntelliQube offers exceptional reproducibility and data quality.

Pipette Wash

The IntelliQube Pipette Wash system reduces tip waste and associated consumable costs. The Pipette Wash uses fluid agitation around the dispense tips to ensure complete interior and exterior cleaning of the tips, while a touch-off feature is used to counteract surface tension and remove residual droplets. Complete elimination of the wash basin contents after each wash cycle combined with the ability to incorporate a wash additive, such as bleach, ensures testing integrity and mitigates the risk of cross-contamination.

Liquid Handling Specifications

Performance chart Sample Dispensing CyBio® FeLiX pipette head Assay Dispensing Dispense Jet
Mechanism Air displacement Single jet solenoid valve
Dispensing configuration 96- or 384-channel 4-channel
Dispense volume 0.8 µL
Precision  ≤5% 
Recommended input Nucleic acid templates 2X Primer+Probe+Mastermix
Source plate positions 10 (ambient temperature) 3 (temperature controlled)
Supported source plates ANSI/SBS compliant 96/384-well formats ≤15 mm in height ANSI/SBS compliant 96-well formats ≤ 30 mm in height
Total dispensing time Approximately 5 to 6 minutes per array

The IntelliQube allows multi-functional dispensing for high speed fluid handling in 384- or 768-well Array Tape®. An interchangeable CyBio FeliX 96- or 384-channel pipetting head precisely dispenses DNA samples into Array Tape.

IntelliQube has an integrated pipette wash system reducing tip waste and associated consumable costs while mitigating the risk of cross contamination.

Amplification 

The IntelliQube integrated amplification is supported by a Peltier thermal cycling block.

  • Designed specifically for 384- and 768-well Array Tape, no block change is required!
  • Temperature uniformity and accuracy enable broad compatibility with amplification methodologies.
  • Excellent ramp rates

Detection

  • An advanced optical system for integrated fluorescence detection
  • Filtered LEDs are optimised for a variety of commonly used fluorophores (range of 480-620 nm)
  • A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm
  • Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds.

Software and data analysis

The Intellics® Software Suite provides:

  • Centralised data management
  • Instrument monitoring
  • Intelligent run optimisation
  • Protocol generation and streamlined data analysis. 

Intellics is pre-loaded on an embedded Windows® operating system, accessible to users through a unified web-based interface.

Intellics Array Builder™ allows users to rapidly generate liquid handling and thermal cycling protocols, and IntelliScore® for post-PCR data analysis and interpretation.

IntelliScore supports absolute quantification (standard curve), relative quantification (relative standard curve and ΔΔCq), genotyping, and presence / absence. CSV data export for LIMS systems is possible.

A touch screen interface facilitates instrument control and also supports real-time data monitoring.

See the benefits that
the IntelliQube system provides

Cq Uniformity

Users can expect minimal well-to-well variability in Array Tape, providing The IntelliQube demonstrated a total standard deviation (SD) of 0.087 cycles when 768 replicate wells each containing 2048 copies of a NIST calibrated human – gDNA reference standard were analyzed for a non- transcribed locus in hgDNA.

intelliqube cq uniformity curve

Figure 1. Cq Uniformity <0.09 SD

Dynamic Range

The IntelliQube can support assays across a large range of input concentrations while maintaining data integrity. When analyzing a 1000 bp synthetic target serially diluted across eight orders of magnitude, the IntelliQube demonstrated a linear dynamic range of quantifications between 4x107 to 40 copies/reaction.

intelliqube dynamic range curve

Figure 2. Linear dynamic range of quantification from 4x107 to 40 copies/reaction

Resolution

Minimal differences in target quality can be accurately resolved between reaction wells on the IntelliQube. Using NIST calibrated hgDNA reference standard, reactions were prepared to represent 1.2 fold differences in template concentration. When analyzed for a non-transcribed locus in hgDNA, the IntelliQube demonstrated the ability to resolve 1.2 fold differences with greater than 97% sensitivity and specificity.

intelliqube fold resolution curve

Figure 3. 1.2 fold resolution

Sensitivity

The IntelliQube provides the sensitivity necessary to amplify and detect even 1-2 copies of target DNA in a reaction well. Although the theoretical limit of detection (LOD) provided by Poisson statistics is limited to three molecules, IntelliQube has demonstrated the ability to detect as little as 1-2 molecules of hgDNA per reaction.

IntelliQube Performance Specifications

Dimensions (W x D x H) 49" x 34" x 75" (with cart)
Weight 855 pounds
Input Voltage 120/240 VAC, 15/7.5 A
Frequency 50-60 Hz
Operating Temperature 15-30°C
Operating Humidity 20-80% Relative Humidity, non-condensing
Air Input 80-100 psi, bursts up to 15 SCFM
Water Input Reverse Osmosis or Deionized
Certifications Conforms to the European Machinery Directive (2006/42/EC) and the EMC Directive (2004/108/EC). For Research Use Only. Not for use in Diagnostic Procedures.
Estimated End-point PCR Throughput Per 8 Hours (With Hydrocycler2) Sixty Five 384-well arrays
24,960 data points

Temperature uniformity

Specification Peltier block type
Block configuration 384/768-well Array Tape
Temperature range 20 - 100°C
Temperature accuracy ±0.25°C
Temperature uniformity ±0.5°C at 60°C
Heating ramp rate 3.0°C/sec
Cooling rate 3.0°C/sec

Detection

LEDs
(460-645 nm)
Dye channel 1 2 3 4 5
Excitation filter (nm) 480 530 548 580 620
Emission Filter (nm) 510 565 580 625 705
Dyes with expected compatability FAM™
JOE™
VIC®/HEX™
Cal Fluor®
Orange 560
NED™
TAMRA™
Cal Fluor® Red 590
ROX™
Cal Fluor® Red 610
Texas Red®
Cy5®
Quasar® 670