KASP primer design and validation
KASP™ has been a go-to choice for genotyping assays by top agriculture breeding programmes worldwide for more than 21 years. KASP is easy to use. Simply add your SNP-specific KASP assay targeting primers (KASP Assay Mix) and the universal KASP-TF Master Mix to your DNA samples then run a thermal cycling program and measure the end-point fluorescence.
If you are new to KASP, start with a free trial kit and get your questions answered by our experts. Once you’ve decided that KASP is a good fit for your application, it’s time to determine how you’d like to leverage our expertise.
- Primer design: We use our proprietary software to select target-specific primers and send you a custom KASP Assay Mix.
- Primer design with validation: In addition to primer selection, our experts can fully wet-lab validate and optimise your assay and send you a fully optimised custom KASP Assay Mix.
- Pre-designed assay libraries: Choose from thousands of pre-validated genotyping assays for several plant and animal species. Learn more.
What is the KASP assay mix?
After you send us your sequence, our in-house experts will use our proprietary software to identify the best mix of three assay-specific non-labelled oligos for your SNP, indel or mega indel:
- Two allele-specific forward primers
- One common reverse primer
Aliquots of your KASP Assay Mix are provided in 2D barcoded tubes in sufficient quantities to enable:
- 2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
- 5,000 reactions at 5 µL total reaction volumes (384-well PCR plates)
Start your KASP journey today by requesting a quote for KASP primer design with optional wet-lab services.
KASP resources
Ordering and project set up
- How to submit sequences
- Sequence submission form
- KASP genotyping protocols for qPCR instruments
- KASP reagents guidance notes to support running KASP genotyping assays in your lab
- KASP assay design - anchoring
- End-point genotyping manual compilation
- KASP trial kit manual
- KASP genotyping manual
- DNA requirements for genotyping services
Blog posts, webinars and case studies
Learn more about applications of KASP genotyping technology.
- Explore the vast applications of KASP genotyping for plant breeding programmes
- Optimise sample collection for aquaculture genotyping
- Increasing resistance to aquaculture disease
- Large-scale genotyping in strawberry genomic selection
- KASP technology accelerates rice improvement in South Asia
- Sustainable pathway for rice growers to meet region demands
Data analysis and troubleshooting
Let's talk about KASP.
We have been the exclusive supplier of KASP genotyping assays for more than 21 years. Whether you need support setting up your KASP genotyping reaction or you’re ready to scale up or diversify your operations, we are here to help.