RNA FISH for cancer targets
Visualisation and quantification of single RNA transcripts, using Stellaris™ RNA fluorescence in situ hybridisation, offers the unique ability to observe altered disease-specific expression in fixed cells or intact tissue
Stellaris RNA FISH, offers the unique ability to observe altered disease-specific expression in fixed cells or intact tissue. Gene expression profiles are complex and can vary immensely between regions of tissue, cells, and even regions of a single nucleus, and therefore require advanced methods of analysis.
Pre-designed cancer sets include:
| ADAM17 | EGFR | HOTAIR | MYC |
| ATK1 | ERBB2&3 | HOXA5 | NCOA3 |
| AR | ERG | JAG1 | PCA3 |
| BCL2 | EZH2 | JUN | PIK3CA |
| BRAF | FOS | KIT | MTOR |
| BRCA1&2 | GREB1 | KLK3 | TERT |
| CDK4 | H19 | MALAT1 | TERC |
| CTNNB1 | HIF1A | MKI67 | TP53 |
Stellaris multiplexing
Image of simultaneous Stellaris RNA FISH and immunofluorescence assay. HER2 mRNA molecules (green), protein marker HER2 (pink), DAPI (red) in human SK-BR-3 cells (AV Orjalo Biosearch Tech.)
Publication highlights
Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science
Visit our Citation Center for the complete list.
Post-transcriptional Modifications Contribute to the Upregulation of Cyclin D2 in Multiple Myeloma.
Misiewicz-Krzeminska et al. Clinical Cancer Research. 2016. url: https://dx.doi.org/10.1158/1078-0432.CCR-14-2796
G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation.
Ruijtenberg et al. Cell. 2015. url: https://dx.doi.org/10.1016/j.cell.2015.06.013
Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA.
Leveille et al. Nature Communications. 2015. url: https://dx.doi.org/10.1038/ncomms7520
Niche appropriation by Drosophila intestinal stem cell tumours.
Patel et al. Nature Cell Biology. 2015. url: https://dx.doi.org/10.1038/ncb3214
FuseFISH: robust detection of transcribed gene fusions in single cells.
Semrau et al. Cell reports. 2014. url: https://dx.doi.org/10.1016/j.celrep.2013.12.002
Proteostatic Control of Telomerase Function through TRiC-Mediated Folding of TCAB1.
Freund et al. Cell. 2014. url: https://dx.doi.org/10.1016/j.cell.2014.10.059
Single-molecule transcript counting of stem-cell markers in the mouse intesting.
Itzkovitz et al. Nature Cell Biology. 2011. url: https://dx.doi.org/10.1038/ncb2384
The Stellaris RNA FISH Method
