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    • Custom DNA probes for qPCR
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  1. Home
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  3. FAQs
  4. qPCR applications, calibration and troubleshooting

qPCR applications, calibration and troubleshooting

How do I adjust my thermal cycler’s settings to account for the BHQ quencher?

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How do I calibrate my instrument for the CAL Fluor and Quasar Dyes?

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I am having a problem with background drift with my BHQ™ Probe. What might the cause be?

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I am not getting any signal generation with my BHQ™ Probe. What might cause this?

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I am performing real time qPCR and my negative controls are amplifying. Why would this happen?

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Why is my BHQ-Pulsar 650 probe not detected in a singleplex reaction on my LightCycler?

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Why do I get different Ct values for the same probe sequence when labelled with a different dye?

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How many PCR reactions will I be able to run with my probe or primer?

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I am a beginner at real-time qPCR. Does Biosearch Technologies have information which will help me to design my assay?

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Does Biosearch Technologies have software available for designing probes and primers?

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Is there a formula for calculating the efficiency of a qPCR reaction?

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Does Biosearch Technologies make available information on multiplex qPCR?

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