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  5. Are KASP assay primers designed to the forward or reverse strand of DNA?

FAQ

Are KASP assay primers designed to the forward or reverse strand of DNA?

For KASP assay design, you can submit the DNA sequence in either orientation. Our Kraken™ software is used for primer design, and will choose the most suitable DNA orientation based on the putative primer properties. The SNP will always be reported to you in the same orientation that you submitted the sequence in.