Which qPCR instruments and plate readers are compatible with KASP?

The qPCR instrument or plate reader must be capable of reading FAM and HEX fluorophores; the relevant excitation and emission values are detailed in the table below.  KASP Master mix also contains ROX, a passive reference dye.  Although it is not essential to read the ROX levels in your reactions, it is advisable to do so as normalisation will improve the quality of the data obtained.

Fluorophore Excitation (nm) Emission (nm)
FAM 485 520
HEX 535 556
ROX 575 610

To view a list of the qPCR instruments that we know work well with KASP, please click here.