What is the minimum number of samples that can be analysed with one KASP assay?

The minimum number of DNA samples recommended for cluster analysis is 22 – this is to ensure that there are sufficient data points for reliable clusters to form.  If you only have a few different DNA samples available, LGC would recommend running the samples in duplicate or triplicate to increase the number of data points in the analysis.  It may also be beneficial to run positive controls of known genotypes in this situation.  At least two no template control should be run for each KASP genotyping assay, giving a minimum of 24 data points per cluster plot.