What is a no template control (NTC) well?

A no template control, typically referred to as an NTC, is a well that is set up in the same way as all DNA sample wells, but does not contain any template DNA.  Sterile water must be used to replace the volume that would be occupied by the wet DNA, as this ensures that the KASP Master mix in the NTC well is diluted from 2X to 1X concentration.  As an example, a 10 µL NTC well would typically contain 5 µL 2X KASP Master mix, 0.14 µL KASP Assay mix and 5 µL sterile water. 

NTC wells are essential for detecting contamination or non-specific amplification, and a minimum of two NTC wells should be included per KASP assay per reaction plate.