To perform KASP genotyping in my own laboratory, what equipment is required?

It is straightforward to perform KASP™ genotyping in your own laboratory. Aside from standard laboratory equipment such as pipettes, all that is additionally required is a thermal cycler and a FRET-capable plate reader, or a qPCR instrument.

The plate reader or qPCR instrument must be capable of reading FAM and HEX fluorophores; the relevant excitation and emission values are detailed in the table below. KASP Master mix also contains ROX, a passive reference dye.  Although it is not essential to read the ROX levels in your reactions, it is advisable to do so as normalisation will improve the quality of the data obtained.

Fluorophore Excitation (nm) Emission (nm)
FAM 485 520
HEX 535 556
ROX 575 610

LGC can supply you with a free-of-charge genotyping trial kit to enable you to try KASP chemistry in your laboratory.