FAQ

The expected coverage depth will depend on the number of samples multiplexed and the number of SNPs being targeted. For a standard service, the minimum coverage to assign a base call is 8x (4x per allele, for a bi-allelic SNP), while a coverage of 20x is recommended to confidently call variants. Depending on the specifics of your project coverage can be as much as (theoretical) 200x, which is influenced by the DNA quality and the efficiency of the library preparation. As there will also be differences in marker coverage (dependent on target region), the oligo characteristics and the total panel composition, we do not guarantee a specific coverage per target.

The final level of coverage, at the bioinformatics stage, is dependent on different factors – it will be a trade-off between excluding more data with potential noise and accepting higher levels of missing data, or including more noise to obtain more (but lower quality) data.