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  6. What factors can cause a Flex-Seq probe design to fail?

FAQ

What factors can cause a Flex-Seq probe design to fail?

The issues that can cause Flex-Seq probe design failure are similar to those for conventional PCR (i.e. high/low GC content, low complexity regions, repetitive motifs flanking the markers). Flex-Seq is significantly more tolerant to polymorphism in the probe binding region, making this factor less of a concern. Therefore a “pre-filter” is included in all Flex-Seq assay design to deselect these regions in order to to increase the success rate for downstream probe binding.