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  6. Can Flex-Seq be used to detect off-target SNPs with CRISPR-Cas9, based on targeting regions of binding site homology (or similar)?

FAQ

Can Flex-Seq be used to detect off-target SNPs with CRISPR-Cas9, based on targeting regions of binding site homology (or similar)?

Yes, Flex-Seq can be used with CRISPR. Please be aware that only the SNPs identified in the design BED file which correlate with the reference genome will be have probes designed for. Flex-Seq will generate sequence data for the full interval between the probes (often 150-250bp) and thus can be used to characterize CRISPER based mutation. Probe design will only be performed on predetermined regions, this means any true off-target SNPs will not be accounted for.