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  5. Can BHQ Probe Master Mix be used in multiplex reactions?

FAQ

Can BHQ Probe Master Mix be used in multiplex reactions?

Yes, RapiDxFire qPCR Master Mix has been optimised for use in multiplex qPCR applications and customers can chose from a wide range of fluorophores from Biosearch Technologies, depending on their qPCR instrument.

When performing multiplexing reactions, we recommend optimising each assay as a singleplex reaction, before combining a multiplex, to evaluate the optimal parameters of each assay (e.g. annealing temperature, cycling conditions etc.)