Workflow

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Figure 1. Fast, simple and user friendly 2-stage protocol in 384-well format. The workflow uses pre-plated reagents and room temperature stable pre-plated indexing plates to drive throughput. The workflow is developed to minimise the impact of user errors, for example through the use of identical PCR cycling conditions. With only one enzyme mix, two reagents and a pre-plated index plate, the reagent system is simple and highly automatable to limit hands-on time.