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Human XIST consists of a set of Quasar® 570-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect XIST transcripts in Human specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against Human X (inactive)-specific transcript (non-protein coding), XIST, a.k.a. XCE; XIC; SXI1; swd66; DXS1089; DXS399E; LINC00001; NCRNA00001 (NCBI gene ID: 7503), and the sequence of NR_001564.2 nts 1-8000.The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of human XIST mRNAs detected with a Quasar 570 dye labeled probe set in MCF7 cells
Properties:
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Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Storage conditions:
Stellaris FISH Probes are shipped dry and may be stored at +2 to +8ºC in this state.
