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  5. Does Biosearch Technologies recommend the use of FAST deprotecting or standard amidites?

FAQ

Does Biosearch Technologies recommend the use of FAST deprotecting or standard amidites?

Biosearch Technologies recommends the use of FAST deprotection amidites such as: Adenosine-benzoyl,A(Bz;) Cytidine-acetyl,C(Ac); Guanosine-dimethylformamide, G(Dmf). For ultra-FAST deprotection, use the phenoxyacetyl (Pac) amidites: A (Pac), C (Pac) and G (Pac). Thymidine does not need a protecting group. The FAST deprotecting amidites are used when dye-labeled oligonucleotides are expected to be sensitive to basic conditions, especially when long time periods of incubation are involved. This is true for the CAL Fluor™ dyes which are known to degrade in concentrated NH4OH.

The use of a tetrazole activator is in concurrence with our chemistry practices. Biosearch Technologies uses ethylthioltetrazol (ETT) for all amidite productions. Be advised that the 4,5-dicyanoimidizole (DCi) activator, while less acidic, more soluble and more nucleophilic than tetrazol or ETT, does NOT work with our CAL Fluor chemistries.