Do I need to run replicates of my DNA samples when performing KASP genotyping?

As analysis of KASP genotyping data is performed using cluster plots, individual DNA samples do not need to be run in duplicate or triplicate – a single data point per DNA sample per KASP assay (SNP / indel) is sufficient.  It is, however, important to meet or exceed the required minimum of 22 DNA samples per KASP assay to ensure that there are sufficient data points for reliable clusters to form.