FAQ

The number of reactions per nmol of product delivered is dependent upon the concentration to be used and final reaction volume. Typically, 1 nmol of a primer designed for qPCR will provide sufficient material for at least 100 reactions if used at a 300 nM final concentration in a 20 µL total volume. Likewise, 1 nmol of dual-labeled BHQ probe will provide sufficient material for up to 500 reactions if used at a 100 nM final concentration in a 20 µL total volume.