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  6. There are multiple SNPs within 100 nt of my SNP of interest. Will the presence of these surrounding SNPs significantly impact oligonucleotide probe design?

FAQ

There are multiple SNPs within 100 nt of my SNP of interest. Will the presence of these surrounding SNPs significantly impact oligonucleotide probe design?

The algorithm software will typically design Flex-Seq targets to be between 200-350 bp in length, avoiding, where possible, repetitive sequences and other oligonucleotide design-limiting variations, including the presence of surrounding SNPs. However, these design constraints can be relaxed, though it should be noted that in highly heterozygous genomes, this may lead to a compromise of target specificity. Please contact technical and applications support regarding the various design options in relation to your Flex-Seq project.