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  6. Can reference scaffolds be used in place of reference chromosome when submitting reference sequences?

FAQ

Can reference scaffolds be used in place of reference chromosome when submitting reference sequences?

Yes, reference scaffolds can be used. Another option would be to use a very closely related species. If either of these options is chosen, it is the responsibility of the customer to ensure that the SNP locations in the BED file match the SNPs in the species of interest.

All Flex-Seq projects require:

  • a reference sequence (genomic or transcriptomic, or a reference scaffold). These can a provided as a link to a publically available genome, or submitted as a FASTA file.
  • a list of SNP locations to be interrogated, submitted as a VCF, Excel or BED file.