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Human POLR2A consists of a set of Quasar® 570-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect POLR2A transcripts in Human specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against Human Polymerase (RNA) II (DNA directed) polypeptide A, 220kD, POLR2A, a.k.a. RPB1; RPO2; POLR2; POLRA; RPOL2 (NCBI gene ID: 5430), and the coding sequence of NM_000937.4 nts 387-6299.The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of Human POLR2A mRNAs detected with a Quasar 570 dye labeled probe set in A549 cells
Properties:
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Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Storage conditions:
Stellaris FISH Probes are shipped dry and may be stored at +2 to +8ºC in this state.
