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Fruit Fly Rpll215 consists of a set of Quasar® 570-labeled oligos at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect Rpll215 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against RNA polymerase II 215kD subunit RpII215 a.k.a. 5; 8WG16; alphaPol IIo[ser2]; CG1554; CTD; Dmel\CG1554; dRpb1; dRPB1; H5; II; IIo; l(1)10Ca; l(1)DC912; l(1)DF912; l(1)G0040; l(1)L5; L5; POL; pol II; Pol II; Pol II CTD; Pol II Ser5p; Pol II Ser5P; Pol II0[ser2]; Pol II0[ser5]; Pol IIa; Pol IIo; Pol IIo[ser2]; Pol IIo[Ser2]; Pol IIo[ser5]; Pol II[ser2]; Pol-IIa; polII; PolII; PolIIa; PolIIo; PolIIo[ser2]; PolIIo[ser5]; RNA pol II; RNA Pol II; RNA Pol II CTD; RNA pol IIo; RNA PolI 215; RNA polII; RNA PolII; RNA-PolII; RNAP; RNAP II; RNAP II LS; RNAPII; RNAPII0; RNApol; RNApol2; RNApolII; Rpb1; RPB1; RpII; rpII1; RPII215; Rpll215; Ser5-P Pol II; Ubl (NCBI gene ID:32100) and nucleotides 406-2965 of NM_078569.3. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Representative image of fruit fly Rpll215 RNAs detected with a Quasar 570 dye labeled probe set in KC cells.
Properties:
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Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
