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Stellaris® FISH Probes, Human PMP22 with Quasar® 670 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect PMP22 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against peripheral myelin protein 22 PMP22 a.k.a. DSS; HNPP; CMT1A; CMT1E; GAS-3; Sp110; HMSNIA (NCBI gene ID:5376) and nucleotides 205-1861 of NM_000304.3. This is an inclusive probe set designed to the detect the following variants: NM_000304.3, NM_153321.2, NM_153322.2, NM_001281455.1, and NM_001281456.1, and partly the minor RNAs: NR_104017.1 and NR_104018.1, as well as the ill supported XM_011523943.1 The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
647
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Fluorescence Maximum:
670
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
