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Stellaris® FISH Probes, Fruit Fly Gapdh1 | 2 with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect Gapdh1 | 2 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against glyceraldehyde-3-phosphate dehydrogenase Gapdh1 | 2 a.k.a. BEST:GH12586; CG12055; Dmel\CG12055; GA3PDH; GADPH; GAP; Gapd; gapdh; Gapdh; GAPDH; GAPDH I; gapdh-1; Gapdh-1; GAPDH-1; GAPDH1; Gapdh43E; GAPDHI; gh12586 | CG8893; Dmel\CG8893; GA3PDH; GADPH; GAP; Gapd; Gapdh; GAPDH; GAPDH II; Gapdh-2; Gapdh13F; GAPDH2 (NCBI gene ID:35728, 32545) and nucleotides 261-1259 of NM_080369.3. This is an inclusive probe set designed to detect the following variants:
Gapdh1: NM_080369.3, NM_001038847.2, and Gapdh2: NM_080714.4, NM_001272655.1. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
569
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Fluorescence Maximum:
591
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
