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Stellaris® FISH Probes, Human ATRX with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect ATRX transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against alpha thalassemia/mental retardation syndrome X-linked ATRX a.k.a. JMS; SHS; XH2; XNP; ATR2; SFM1; RAD54; MRXHF1; RAD54L; ZNF-HX (NCBI gene ID:546) and nucleotides 402-638, 642-1871, 1959-572 of NM_138270.3. This is an inclusive probe set designed to also detect the following variants: NM_000489, XM_005262153, XM_005262154, XM_005262155, XM_005262156, XM_005262157, XM_006724666, XM_006724667, XM_006724668. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
569
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Fluorescence Maximum:
591
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
